Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

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Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis.The 2-DE patterns of proteins showed qualitative differences between plants propagated in Preserve vitro and control seedlings.More proteins of a high molecular weight were observed in leaves of plants from in vitro culture.

A two-dimensional map of proteins from leaves Windowgrill Standing Mirror provides detailed data concerning both polymorphism and protein patterns of this species.This makes it possible to start constructing a protein map of R.chamaemorus.

The reasons for qualitative differences are discussed.